Fig 2: Hypoixa inhibited the mTOR pathway and AMPK activation, and propofol attenuated these inhibitory effects via regulation of BTG3. PC-12 cells were divided into four groups, including Normoxia, Hypoxia, Propofol, and Hypoxia + propofol. a Protein expression of BTG3 by Western blot analysis. Phosphorylated levels of key kinases in the mTOR pathway (b) and AMPK (c) were assessed by Western blot analysis

Fig 3: BTG3 overexpression inhibited the mTOR pathway and AMPK activation in PC-12 cells. a Protein expression of BTG3 was assessed by Western blot analysis. Phosphorylated levels of key kinases in the mTOR pathway (b) and AMPK (c) were assessed by Western blot analysis. PC-12 cells were transfected with pcDNA3.1, pc-BTG3, sh-NC or sh-BTG3. Data was presented as the mean ± standard deviation (SD) of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001